sterol

Cell culture and plasmid DAP (triple affinity-purification tag; a biotinylation sequence and FLAG tags, and N-terminal epitope tag; 6-histidine epitope tag) -TDP-43-inducible 293 T Rex cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, glucose, 4.5 g/l) supplemented with Tet System Approved FBS (Takara Bio USA, CA, USA) at 37 °C in […]